Palavras-chave
verificação
separação imunomagnética
quantificação
exatidão
recuperação
linearidade
Como Citar
Resumo
O Real Decreto 865/2003 para a prevenção e controle da legionelose menciona o método de cultivo da norma ISO 11731 para o teste de Legionella spp., uma norma em torno da qual são definidos limites paramétricos. As fragilidades observadas nesta ISO (falsos negativos e longos tempos de espera) obrigaram à procura de métodos alternativos. Estuda-se, se o método de separação imunomagnética e quantificação por colorimetria de Legionella spp., Legipid® Legionella Fast Detection, (SIM), é adequado e eficiente num laboratório oficial de Saúde Pública, para amostras ambientais de água. Na ausência de uma metodologia padronizada para verificar métodos alternativos, desenhou-se um método para realizar essa verificação, tendo em conta os critérios específicos para análises microbiológicas e o guia ENAC para kits de teste. Assim, foram calculados os parâmetros de precisão e recuperação nas amostras inoculadas e, adicionalmente, a linearidade da inclinação e da ordenada na origem da relação entre absorbância e unidades formadoras de colônia, comparando os dados experimentais obtidos com os resultados de método de validação. Os resultados foram satisfatórios em todos os casos. Uma dispersão inferior a 20 %, uma recuperação de 4 % - 71 % e uma linearidade de acordo com a validação a um nível de significância superior a 95 %. Com base nesses resultados, o método SIM é considerado um método válido para a deteção e quantificação de Legionella spp. num laboratório oficial de saúde pública.Referências
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